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Addgene

pET15b (I-WcaINhis) K125I
(Plasmid #195619)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195619 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-15b
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    I-WcaI
  • Species
    Other
  • Mutation
    A2030T, A2031T
  • Promoter T7

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expresses the I-WcaI homing endonuclease from Wickerhamomyces canadensis. The sequence is codon optimized for expression in E. coli. The expressed protein contains a K125I mutation that results in DNA nicking activity. It contains an amino-terminal histidine tag for purification.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b (I-WcaINhis) K125I was a gift from Frederick Gimble (Addgene plasmid # 195619 ; http://n2t.net/addgene:195619 ; RRID:Addgene_195619)
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