pET15b I-SceI (Ctermhis -MH)
(Plasmid #195613)

• Purpose: Expresses the wild-type I-SceI homing endonuclease from the T7 promoter
• Depositing Lab: Frederick Gimble
• Publication: Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202. doi: 10.1016/j.jmb.2008.07.010. Epub 2008 Jul 11.

Backbone

• Vector backbone: pET-15b
• Backbone manufacturer: Novagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: I-SceI
• Species: S. cerevisiae (budding yeast)
• Mutation: None
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Originally derived from pSCM525, a plasmid from Dr. Bernard Dujon at the Institute Pasteur.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Expresses the I-SceI homing endonuclease under the control of the phage T7 promoter. The protein contains a carboxyl-terminal histidine tag for purification. The sequence is codon optimized for expression in E. coli and is derived from plasmid pSCM525 (Dr. Bernard Dujon, Institut Pasteur).