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Addgene

pCMV_NOPlight1
(Plasmid #195578)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195578 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Backbone size w/o insert (bp) 3986
  • Total vector size (bp) 5909
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NOPLight1
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1923
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer ggatagcggtttgactcacggggatttcc
  • 3′ sequencing primer ccctatctcggtctattcttttgatttataagg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV_NOPlight1 was a gift from Tommaso Patriarchi (Addgene plasmid # 195578 ; http://n2t.net/addgene:195578 ; RRID:Addgene_195578)

  • For your References section:

    Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide release. Zhou X, Stine C, Prada PO, Fusca D, Assoumou K, Dernic J, Bhat MA, Achanta AS, Johnson JC, Pasqualini AL, Jadhav S, Bauder CA, Steuernagel L, Ravotto L, Benke D, Weber B, Suko A, Palmiter RD, Stoeber M, Kloppenburg P, Bruning JC, Bruchas MR, Patriarchi T. Nat Commun. 2024 Jun 25;15(1):5353. doi: 10.1038/s41467-024-49712-0. 10.1038/s41467-024-49712-0 PubMed 38918403
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