attB-P7-attP-YFP
(Plasmid #195571)

• Purpose: A strong P7 promoter flanked by Bxb1 recombination sites attB and attP. Downstream of attP is a strong RBS and YFP coding sequence.
• Depositing Lab: Richard Murray
• Publication: Pandey et al ACS Synth Biol. 2023 Feb 17;12(2):511-523. doi: 10.1021/acssynbio.2c00534. Epub 2023 Jan 30.

Backbone

• Vector backbone: pSC101
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Promoter flanked by Bxb1 recombination sites attB and attP with YFP downstream
• Species: Synthetic
• Insert Size (bp): 4656
• Promoter: P7

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AGCTCCGGCAAAAAAACGGGCAAGG
• 3′ sequencing primer: ATTACCGCCTTTGAGTGAGC

Resource Information

• Supplemental Documents:
  • attB-p7-attP flagged YFP Reporter - Victoria's Archive (pVHct1Y).gb
• A portion of this plasmid was derived from a plasmid made by: Dr. Victoria Hsiao originally cloned it at Murray lab, Caltech for this preprint: https://www.biorxiv.org/content/10.1101/121152v3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Dr. Victoria Hsiao originally cloned this plasmid at Murray lab, Caltech for this preprint: https://www.biorxiv.org/content/10.1101/121152v3

We use this plasmid for in vitro cell-free protein expression system for our paper.