pAR010 Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD
(Plasmid
#195543)
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PurposedCas12a effector fusion for manipulating transcription
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195543 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneunknown
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD
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SpeciesH. sapiens (human)
- Promoter pEF1a
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Tag
/ Fusion Protein
- 3XHA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.05.12.540558v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAR010 Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD was a gift from Michael Bassik (Addgene plasmid # 195543 ; http://n2t.net/addgene:195543 ; RRID:Addgene_195543) -
For your References section:
Development of compact transcriptional effectors using high-throughput measurements in diverse contexts. Tycko J, Van MV, A, DelRosso N, Yao D, Xu X, Ludwig C, Spees K, Liu K, Hess GT, Gu M, Mukund AX, Suzuki PH, Kamber RA, Qi LS, Bintu LB, Bassik MC.. bioRxiv. (PrePrint) 10.1101/2023.05.12.540558