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Addgene

pAAV-CAG-DIO-VARNAM2-Kv2.1PR
(Plasmid #195533)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195533 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-CAG
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VARNAM2-Kv2.1 proximal restriction sequence
  • Species
    Synthetic
  • Mutation
    VARNAM SI linker
  • GenBank ID
    OM687164
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (unknown if destroyed)
  • 3′ cloning site NheI (unknown if destroyed)
  • 5′ sequencing primer CTCTAGAGCCTCTGCTAACC
  • 3′ sequencing primer GGCCACAACTCCTCATAAAGAGACAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CAG-DIO-VARNAM2-Kv2.1PR was a gift from Vincent Pieribone (Addgene plasmid # 195533 ; http://n2t.net/addgene:195533 ; RRID:Addgene_195533)

  • For your References section:

    Dual-polarity voltage imaging of the concurrent dynamics of multiple neuron types. Kannan M, Vasan G, Haziza S, Huang C, Chrapkiewicz R, Luo J, Cardin JA, Schnitzer MJ, Pieribone VA. Science. 2022 Nov 4;378(6619):eabm8797. doi: 10.1126/science.abm8797. Epub 2022 Nov 4. 10.1126/science.abm8797 PubMed 36378956
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