pAAV-CNTF-HA
(Plasmid #195517)

• Purpose: Expresses HA-tagged CNTF in mammalian cells
• Depositing Lab: Kevin Park
• Publication: Ribeiro et al Exp Neurol. 2022 Sep;355:114147. doi: 10.1016/j.expneurol.2022.114147. Epub 2022 Jun 20.

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Backbone

• Vector backbone: pAAV-MCS
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4650
• Total vector size (bp): 6052
• Modifications to backbone: Ribeiro M, Ayupe AC, Beckedorff FC, Levay K, Rodriguez S, Tsoulfas P, Lee JK, Nascimento-dos-Santos G, Park KK. Retinal ganglion cell expression of cytokine enhances occupancy of NG2 cell-derived astrocytes at the nerve injury site: Implication for axon regeneration
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Ciliary neurotrophic factor
• Alt name: CNTF
• Species: H. sapiens (human)
• Insert Size (bp): 651
• GenBank ID: NM_000614.4
• Entrez Gene: CNTF (a.k.a. HCNTF)
• Promoter: UbC promoter with beta-globin intron
• Tags / Fusion Proteins:
  • NGF signal peptide (N terminal on insert)
  • HA-tag (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: caacctggactctgcggatg
• 3′ sequencing primer: catcccatccgcagagtcc

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry