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pBbA5k-acrAB-sfGFP
(Plasmid #195516)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195516 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBbA5k
  • Backbone size w/o insert (bp) 3658
  • Total vector size (bp) 8769
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BW25113
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    acrAB
  • Species
    E. coli
  • Insert Size (bp)
    5111
  • Promoter lacUV5
  • Tag / Fusion Protein
    • sfgfp (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ggaattgtgagcggataacaatttc
  • 3′ sequencing primer cgttttatttgatgcctggagatcc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBbA5k-acrAB-sfGFP was a gift from Mary Dunlop (Addgene plasmid # 195516 ; http://n2t.net/addgene:195516 ; RRID:Addgene_195516)

  • For your References section:

    Antibiotic export by efflux pumps affects growth of neighboring bacteria. Wen X, Langevin AM, Dunlop MJ. Sci Rep. 2018 Oct 11;8(1):15120. doi: 10.1038/s41598-018-33275-4. 10.1038/s41598-018-33275-4 PubMed 30310093
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