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Addgene

pFastBac1 Flag Baf170
(Plasmid #1955)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1955 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4775
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BAF170
  • Species
    H. sapiens (human)
  • Entrez Gene
    SMARCC2 (a.k.a. BAF170, CRACC2, CSS8, Rsc8)
  • Tag / Fusion Protein
    • flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer Polyhedrin forward
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pBS(SK-)-BAF170 (T3) from Drs. Weidong Wang and Gerald Crabtree

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To make pFastBac1-BAF170, BAF170 sequences were transferred into pFastBac1 using NotI and HindIII. A C-terminal Flag epitope was added by PCR. The product of this PCR reaction was digested with SphI and HindIII, transferred into pFastBac1-BAF170. (kingston #1232)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac1 Flag Baf170 was a gift from Robert Kingston (Addgene plasmid # 1955 ; http://n2t.net/addgene:1955 ; RRID:Addgene_1955)

  • For your References section:

    Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits. Phelan ML, Sif S, Narlikar GJ, Kingston RE. Mol Cell 1999 Feb;3(2):247-53. 10.1016/S1097-2765(00)80315-9 PubMed 10078207
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