pGL4.27_eSOX17.2
(Plasmid #195499)

• Purpose: Firefly luciferase enhancer expression vector driven by a minimal CMV-promoter and the core endoderm distal enhancer of human SOX17
• Depositing Lab: Alexander Meissner
• Publication: Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.7554/eLife.83077.

Backbone

• Vector backbone: pGL4.27
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 6010
• Total vector size (bp): 6645
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Core distal endoderm enhancer region of SOX17
• Alt name: eSOX17.2
• Species: H. sapiens (human)
• Insert Size (bp): 635
• GenBank ID: hg19, chr8:55137558-55138192
• Entrez Gene: SOX17 (a.k.a. VUR3)
• Tag / Fusion Protein:
  • minP_luc2-hPEST (C terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry