pGL4.15_pSOX17
(Plasmid
#195496)
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PurposeFirefly luciferase promoter expression vector driven only by the promoter element of human SOX17
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195496 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL4.15
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5969
- Total vector size (bp) 11019
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePromoter region of SOX17
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Alt namepSOX17
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SpeciesH. sapiens (human)
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Insert Size (bp)5050
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GenBank IDhg19, chr8:55365464-55370513
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Entrez GeneSOX17 (a.k.a. VUR3)
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Tag
/ Fusion Protein
- luc2-hPEST (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL4.15_pSOX17 was a gift from Alexander Meissner (Addgene plasmid # 195496 ; http://n2t.net/addgene:195496 ; RRID:Addgene_195496) -
For your References section:
T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation. Landshammer A, Bolondi A, Kretzmer H, Much C, Buschow R, Rose A, Wu HJ, Mackowiak SD, Braendl B, Giesselmann P, Tornisiello R, Parsi KM, Huey J, Mielke T, Meierhofer D, Maehr R, Hnisz D, Michor F, Rinn JL, Meissner A. Elife. 2023 Jan 31;12:e83077. doi: 10.7554/eLife.83077. 10.7554/eLife.83077 PubMed 36719724