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Addgene

pInducer 21-3xFlag-hMEFV-ΔPLD
(Plasmid #195479)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195479 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pInducer21
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MEFV
  • Alt name
    MEditerranean FeVer gene
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2217
  • Mutation
    deletion of residues 88 - 310 (the resulting pyrin protein lacks the Phosphorylated Linker Domain domain)
  • Entrez Gene
    MEFV (a.k.a. FMF, MEF, PAAND, TRIM20)
  • Tag / Fusion Protein
    • 3xFlag (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer gtccagtgtggtgga
  • 3′ sequencing primer GCCACTGTGCTGGATATCA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pInducer 21-3xFlag-hMEFV-ΔPLD was a gift from Thomas Henry (Addgene plasmid # 195479 ; http://n2t.net/addgene:195479 ; RRID:Addgene_195479)

  • For your References section:

    Mutations in the B30.2 and the central helical scaffold domains of pyrin differentially affect inflammasome activation. Chirita D, Bronnec P, Magnotti F, Dalmon S, Martin A, Popoff M, Gerfaud-Valentin M, Seve P, Belot A, Contis A, Duquesne A, Nocturne G, Lemelle I, Georgin-Lavialle S, Boursier G, Touitou I, Jamilloux Y, Henry T. Cell Death Dis. 2023 Mar 25;14(3):213. doi: 10.1038/s41419-023-05745-9. 10.1038/s41419-023-05745-9 PubMed 36966139
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