pMZ-432
(Plasmid
#195463)
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PurposeLentiviral Integratable, 4OHT/TMX- and GZV-controllable expression of synZiFTR: ZF3-p65
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195463 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHR
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepSFFV - SMASh - ZF3 - p65 - ERT2
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
L236P mutation in SMASh tag does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMZ-432 was a gift from Ahmad Khalil (Addgene plasmid # 195463 ; http://n2t.net/addgene:195463 ; RRID:Addgene_195463) -
For your References section:
Multidimensional control of therapeutic human cell function with synthetic gene circuits. Li HS, Israni DV, Gagnon KA, Gan KA, Raymond MH, Sander JD, Roybal KT, Joung JK, Wong WW, Khalil AS. Science. 2022 Dec 16;378(6625):1227-1234. doi: 10.1126/science.ade0156. Epub 2022 Dec 15. 10.1126/science.ade0156 PubMed 36520914