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pHAGE-N'-FLAG-HA-NAP1S318A
(Plasmid #195410)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195410 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE-N-FLAG-HA-IRES-PURO
  • Backbone manufacturer
    Gateway, backbone originally from J.Wade Harper lab
  • Backbone size w/o insert (bp) 9613
  • Total vector size (bp) 9978
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nak associated protein 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1179
  • Mutation
    Serine 318 turned to Alanine
  • Entrez Gene
    AZI2 (a.k.a. AZ2, NAP1, TILP)
  • Tag / Fusion Protein
    • FLAG-HA (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-N'-FLAG-HA-NAP1S318A was a gift from Alicia Pickrell (Addgene plasmid # 195410 ; http://n2t.net/addgene:195410 ; RRID:Addgene_195410)

  • For your References section:

    NAK-associated protein 1/NAP1 activates TBK1 to ensure accurate mitosis and cytokinesis. Paul S, Sarraf SA, Nam KH, Zavar L, DeFoor N, Biswas SR, Fritsch LE, Yaron TM, Johnson JL, Huntsman EM, Cantley LC, Ordureau A, Pickrell AM. J Cell Biol. 2024 Feb 5;223(2):e202303082. doi: 10.1083/jcb.202303082. Epub 2023 Dec 7. 10.1083/jcb.202303082 PubMed 38059900
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