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W279A-short-UBASH3A-V5-6xHis
(Plasmid #195409)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195409 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF-DEST51
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    UBASH3A
  • Species
    H. sapiens (human)
  • Mutation
    W279A
  • Entrez Gene
    UBASH3A (a.k.a. TULA, CLIP4, STS-2)
  • Tag / Fusion Protein
    • V5-6xHis (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    W279A-short-UBASH3A-V5-6xHis was a gift from Patrick Concannon (Addgene plasmid # 195409 ; http://n2t.net/addgene:195409 ; RRID:Addgene_195409)

  • For your References section:

    UBASH3A Regulates the Synthesis and Dynamics of TCR-CD3 Complexes. Ge Y, Paisie TK, Chen S, Concannon P. J Immunol. 2019 Dec 1;203(11):2827-2836. doi: 10.4049/jimmunol.1801338. Epub 2019 Oct 28. 10.4049/jimmunol.1801338 PubMed 31659016
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