deltaUBA-short-UBASH3A-V5-6xHis
(Plasmid
#195404)
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PurposeMammalian expression of human short-form UBASH3A lacking the UBA domain with V5-6xHis tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195404 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEF-DEST51
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUBASH3A
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SpeciesH. sapiens (human)
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MutationUBASH3A mutant lacking the UBA domain
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Entrez GeneUBASH3A (a.k.a. TULA, CLIP4, STS-2)
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Tag
/ Fusion Protein
- V5-6xHis (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
deltaUBA-short-UBASH3A-V5-6xHis was a gift from Patrick Concannon (Addgene plasmid # 195404 ; http://n2t.net/addgene:195404 ; RRID:Addgene_195404)