pGEX-6P-1-PDE-short-UBASH3A
(Plasmid
#195403)
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PurposeBacterial expression of a putative PDE domain of human short-form UBASH3A with GST tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195403 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-6P-1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUBASH3A
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SpeciesH. sapiens (human)
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MutationPDE domain of UBASH3A
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Entrez GeneUBASH3A (a.k.a. TULA, CLIP4, STS-2)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-6P-1-PDE-short-UBASH3A was a gift from Patrick Concannon (Addgene plasmid # 195403 ; http://n2t.net/addgene:195403 ; RRID:Addgene_195403)