pFastBac1 Flag BAF155 b
(Plasmid #1954)

• Depositing Lab: Robert Kingston
• Publication: Phelan et al Mol Cell 1999 Feb;3(2):247-53.

Backbone

• Vector backbone: pFastBac1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4775
• Vector type: Insect Expression
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BAF155
• Alt name: SWI3
• Species: H. sapiens (human)
• Insert Size (bp): 4200
• Entrez Gene: SMARCC1 (a.k.a. BAF155, CRACC1, Rsc8, SRG3, SWI3)
• Tag / Fusion Protein:
  • flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI/XhoI (destroyed during cloning)
• 3′ cloning site: SphI (not destroyed)
• 5′ sequencing primer: Polyhedrin forward

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

We constructed a composite full-length cDNA for hSWI3 (BAF155) using a 2.4 kb partial human cDNA that was isolated as described previously and a 1.8 kb RT-PCR-amplified fragment obtained from human liver poly(A) RNA. We consistently isolated the partial hSWI3 cDNA (2.4 kb) from several libraries. It appears that an adenine-rich stretch at the 3' end of these cDNAs hybridizes with the oligo(dt) primers used to create the first strand of the cDNA. An oligo that anneals in the sense direction to the partial cDNA, and an oligo that anneals to the 3' UTR of the highly related hSWI3 clone described previously, BAF155, were used to generate the missing coding sequences. The amplified fragment was inserted into the BamHI and SpeI sites of pBS-5'hSWI3, which contains the partial hSWI3 cDNA. The protein encoded by this complete ORF is highly related to BAF155, except for a few positions scattered throughout the protein. Most of these differences are substitutions of BAF155 sequences with the corresponding sequences of BAF170. To generate pFastBac1-BAF155, an XhoI