FLAG-Ex1-17-deltaP1P2-PTPN22
(Plasmid
#195391)
-
PurposeMammalian expression of a human PTPN22 mutant (exon 1-17 without the P1 and P2 domains) with FLAG tag
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 195391 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCMV-Tag2
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namePTPN22
-
SpeciesH. sapiens (human)
-
MutationPTPN22 mutant containing exon 1 to 17 and lacking the P1 and P2 proline-rich domains
-
Entrez GenePTPN22 (a.k.a. LYP, LYP1, LYP2, PEP, PTPN22.5, PTPN22.6, PTPN8)
-
Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Resource Information
-
Addgene Notes
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
FLAG-Ex1-17-deltaP1P2-PTPN22 was a gift from Patrick Concannon (Addgene plasmid # 195391 ; http://n2t.net/addgene:195391 ; RRID:Addgene_195391)