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Addgene

FLAG-Ex1-17-PTPN22
(Plasmid #195375)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195375 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Tag2
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PTPN22
  • Species
    H. sapiens (human)
  • Mutation
    PTPN22 mutant containing exon 1 to 17
  • Entrez Gene
    PTPN22 (a.k.a. LYP, LYP1, LYP2, PEP, PTPN22.5, PTPN22.6, PTPN8)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-Ex1-17-PTPN22 was a gift from Patrick Concannon (Addgene plasmid # 195375 ; http://n2t.net/addgene:195375 ; RRID:Addgene_195375)

  • For your References section:

    Targeted Deep Sequencing in Multiple-Affected Sibships of European Ancestry Identifies Rare Deleterious Variants in PTPN22 That Confer Risk for Type 1 Diabetes. Ge Y, Onengut-Gumuscu S, Quinlan AR, Mackey AJ, Wright JA, Buckner JH, Habib T, Rich SS, Concannon P. Diabetes. 2016 Mar;65(3):794-802. doi: 10.2337/db15-0322. Epub 2015 Dec 2. 10.2337/db15-0322 PubMed 26631741
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