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Addgene

hfCas12Max
(Plasmid #195336)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195336 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    px330
  • Backbone manufacturer
    Feng Zhang (Addgene plasmid # 42230)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human codon-optimized hfCas12Max
  • Species
    Synthetic
  • Mutation
    N243R, E336R, D892R
  • Promoter CBh, CMV, hU6
  • Tag / Fusion Protein
    • 3xFlag (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hfCas12Max was a gift from Yingsi Zhou (Addgene plasmid # 195336 ; http://n2t.net/addgene:195336 ; RRID:Addgene_195336)

  • For your References section:

    An engineered xCas12i with high activity, high specificity, and broad PAM range. Zhang H, Kong X, Xue M, Hu J, Wang Z, Wei Y, Wang H, Zhou J, Zhang W, Xu M, Shen X, Yin F, Ai Z, Huang G, Xia J, Song X, Li H, Yuan Y, Li J, Zhong N, Zhang M, Zhou Y, Yang H. Protein Cell. 2023 Jun 28;14(7):538-543. doi: 10.1093/procel/pwac052. 10.1093/procel/pwac052 PubMed 37378659
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