pGMC00018 (aka pRC0082)
(Plasmid
#195320)
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Purpose(Empty Backbone) Minimal plasmid backbone with multiple cloning site for HDR donor DNA construction
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195320 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneBPK3082
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Modifications to backboneModified vector BPK3082 (Plasmid #78742) by removing guide RNA cassette and replacing with multiple cloning site
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Vector typeMammalian Expression, Bacterial Expression, CRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGMC00018 (aka pRC0082) was a gift from Raj Chari (Addgene plasmid # 195320 ; http://n2t.net/addgene:195320 ; RRID:Addgene_195320)