pGMC00026 (aka pRC0462)
(Plasmid
#195318)
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Purpose(Empty Backbone) Lentiviral guide RNA backbone derived from pSB700 with neomycin/G418
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195318 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSB700
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Modifications to backboneReplaced mCerulean with Neomycin/G418
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGMC00026 (aka pRC0462) was a gift from Raj Chari (Addgene plasmid # 195318 ; http://n2t.net/addgene:195318 ; RRID:Addgene_195318)