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Addgene

pGMC00024 (aka pCW-Cas9n-Blast)
(Plasmid #195315)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195315 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCW-Cas9-Blast
  • Modifications to backbone
    Modified Cas9 to Cas9 nickase
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-nickase
  • Species
    H. sapiens (human), Synthetic
  • Mutation
    D10A nickase

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGMC00024 (aka pCW-Cas9n-Blast) was a gift from Raj Chari (Addgene plasmid # 195315 ; http://n2t.net/addgene:195315 ; RRID:Addgene_195315)
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