pOPO635
(Plasmid #195297)

• Purpose: pACYC-plac-MycaTnsABC, lac promoter expressing transposase genes TnsABC of McCAST (Tn7575).
• Depositing Lab: Joseph Peters
• Publication: Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765-782. doi: 10.1093/nar/gkac1216.

Backbone

• Vector backbone: pACYC-plac
• Backbone size w/o insert (bp): 2430
• Total vector size (bp): 6278
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TnsABC operon of McCAST (Tn7575)
• Species: Myxacorys californica WJT36-NPBG1
• Insert Size (bp): 3910
• GenBank ID: JAHHHO010000011
• Promoter: IPTG inducible lac promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (destroyed during cloning)
• 3′ cloning site: BsaI (destroyed during cloning)
• 5′ sequencing primer: gtaaaacgacggccagt
• 3′ sequencing primer: caggaaacagctatgac

Resource Information

• Supplemental Documents:
  • pTwist-MycaABC.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry