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PB-PG13-mCherry-EF1a-PuroR-EGFP
(Plasmid #195292)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195292 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB-EF1a-PuroR-EGFP
  • Backbone manufacturer
    H. Oguro, unpublished
  • Total vector size (bp) 11238
  • Vector type
    Mammalian Expression ; piggyBac transposon
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    711

Gene/Insert 2

  • Gene/Insert name
    PuroR-P2A-EGFP
  • Alt name
    Puromycin-N-acetyltransferase
  • Alt name
    Enhanced green fluorescent protein
  • Species
    Synthetic
  • Insert Size (bp)
    1389

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was generated by inserting the PG13-mCherry sequence (Addgene 195291) into the PB-EF1a-PuroR-EGFP plasmid (H. Oguro, unpublished), and contains the piggyBac 3'-inverted terminal repeat (PB 3'ITR), 5'-HS4 chicken b-globin (cHS4) insulator, PG13-mCherry reporter, woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), bovine growth hormone polyadenylation signal (bGHpA), EF1a promoter, puromycin registrant gene, P2A peptide, EGFP, bGHpA, cHS4 insulator, and PB 5'ITR, in this order.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-PG13-mCherry-EF1a-PuroR-EGFP was a gift from Hideyuki Oguro (Addgene plasmid # 195292 ; http://n2t.net/addgene:195292 ; RRID:Addgene_195292)

  • For your References section:

    A reference human induced pluripotent stem cell line for large-scale collaborative studies. Pantazis CB, Yang A, Lara E, McDonough JA, Blauwendraat C, Peng L, Oguro H, Kanaujiya J, Zou J, Sebesta D, Pratt G, Cross E, Blockwick J, Buxton P, Kinner-Bibeau L, Medura C, Tompkins C, Hughes S, Santiana M, Faghri F, Nalls MA, Vitale D, Ballard S, Qi YA, Ramos DM, Anderson KM, Stadler J, Narayan P, Papademetriou J, Reilly L, Nelson MP, Aggarwal S, Rosen LU, Kirwan P, Pisupati V, Coon SL, Scholz SW, Priebe T, Ottl M, Dong J, Meijer M, Janssen LJM, Lourenco VS, van der Kant R, Crusius D, Paquet D, Raulin AC, Bu G, Held A, Wainger BJ, Gabriele RMC, Casey JM, Wray S, Abu-Bonsrah D, Parish CL, Beccari MS, Cleveland DW, Li E, Rose IVL, Kampmann M, Calatayud Aristoy C, Verstreken P, Heinrich L, Chen MY, Schule B, Dou D, Holzbaur ELF, Zanellati MC, Basundra R, Deshmukh M, Cohen S, Khanna R, Raman M, Nevin ZS, Matia M, Van Lent J, Timmerman V, Conklin BR, Johnson Chase K, Zhang K, Funes S, Bosco DA, Erlebach L, Welzer M, Kronenberg-Versteeg D, Lyu G, Arenas E, Coccia E, Sarrafha L, Ahfeldt T, Marioni JC, Skarnes WC, Cookson MR, Ward ME, Merkle FT. Cell Stem Cell. 2022 Dec 1;29(12):1685-1702.e22. doi: 10.1016/j.stem.2022.11.004. 10.1016/j.stem.2022.11.004 PubMed 36459969
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