pFB2F
(Plasmid
#195289)
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PurposeBicistronic HSV-TK and mScarlet resistance for positive/negative selection
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195289 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDTA
- Backbone size w/o insert (bp) 3765
- Total vector size (bp) 5652
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHSV-TK 2A mScarlet
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SpeciesSynthetic
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Insert Size (bp)2004
- Promoter PGK
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M1 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFB2F was a gift from Scott Lowe (Addgene plasmid # 195289 ; http://n2t.net/addgene:195289 ; RRID:Addgene_195289) -
For your References section:
MACHETE identifies interferon-encompassing chromosome 9p21.3 deletions as mediators of immune evasion and metastasis. Barriga FM, Tsanov KM, Ho YJ, Sohail N, Zhang A, Baslan T, Wuest AN, Del Priore I, Meskauskaite B, Livshits G, Alonso-Curbelo D, Simon J, Chaves-Perez A, Bar-Sagi D, Iacobuzio-Donahue CA, Notta F, Chaligne R, Sharma R, Pe'er D, Lowe SW. Nat Cancer. 2022 Nov;3(11):1367-1385. doi: 10.1038/s43018-022-00443-5. Epub 2022 Nov 7. 10.1038/s43018-022-00443-5 PubMed 36344707