pFB2A
(Plasmid
#195283)
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PurposeBicistronic DTR and Hygromycin resistance for positive/negative selection
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195283 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDTA
- Backbone size w/o insert (bp) 3566
- Total vector size (bp) 5279
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDTR 2A Hygro
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Alt nameHB-EGF
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)1713
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Entrez GeneHBEGF (a.k.a. DTR, DTS, DTSF, HEGFL)
- Promoter PGK
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFB2A was a gift from Scott Lowe (Addgene plasmid # 195283 ; http://n2t.net/addgene:195283 ; RRID:Addgene_195283) -
For your References section:
MACHETE identifies interferon-encompassing chromosome 9p21.3 deletions as mediators of immune evasion and metastasis. Barriga FM, Tsanov KM, Ho YJ, Sohail N, Zhang A, Baslan T, Wuest AN, Del Priore I, Meskauskaite B, Livshits G, Alonso-Curbelo D, Simon J, Chaves-Perez A, Bar-Sagi D, Iacobuzio-Donahue CA, Notta F, Chaligne R, Sharma R, Pe'er D, Lowe SW. Nat Cancer. 2022 Nov;3(11):1367-1385. doi: 10.1038/s43018-022-00443-5. Epub 2022 Nov 7. 10.1038/s43018-022-00443-5 PubMed 36344707