pCMV-T7-ABEmax-SpRY
(Plasmid #195279)

• Purpose: Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFP
• Depositing Labs: Carsten Lederer, Petros Patsali
• Publication: pCMV-T7-ABEmax editors (unpublished)

Backbone

• Vector backbone: pCMV
• Backbone manufacturer: David Liu (Addgene plasmid # 112093)
• Backbone size w/o insert (bp): 3393
• Total vector size (bp): 8811
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human codon optimized ABEmax(7.10) SpCas9 variant named SpRY without EGFP
• Species: Synthetic
• Insert Size (bp): 5418
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: M13 Rev

Resource Information

• Supplemental Documents:
  • pCMV_T7_ABEmax(7.10)_SpRY.gbk
• A portion of this plasmid was derived from a plasmid made by: Constructed in the Molecular Genetics Thalassemia Department by Basma Naiisseh using the following plasmids from Addgene: # 140002 and 140003, as well as # 112093.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry