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Addgene

lenti-fullef1a-FAKSPARK2
(Plasmid #195265)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195265 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lenti vector
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FAKSPARK2 sensor
  • Insert Size (bp)
    2356
  • Promoter ef1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (destroyed during cloning)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer ef1aF
  • 3′ sequencing primer wprer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lenti-fullef1a-FAKSPARK2 was a gift from Xiaokun Shu (Addgene plasmid # 195265 ; http://n2t.net/addgene:195265 ; RRID:Addgene_195265)

  • For your References section:

    Polarized focal adhesion kinase activity within a focal adhesion during cell migration. Li X, Combs JD 3rd, Salaita K, Shu X. Nat Chem Biol. 2023 Jun 22. doi: 10.1038/s41589-023-01353-y. 10.1038/s41589-023-01353-y PubMed 37349581
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