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ssFv_lenti_wtATMSPARK2
(Plasmid #195249)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195249 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lenti vector
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATMSPARK2 sensor
  • Insert Size (bp)
    2505
  • Promoter ssfv

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer ssfv_forward
  • 3′ sequencing primer wprer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ssFv_lenti_wtATMSPARK2 was a gift from Xiaokun Shu (Addgene plasmid # 195249 ; http://n2t.net/addgene:195249 ; RRID:Addgene_195249)

  • For your References section:

    ATM-SPARK: A GFP phase separation-based activity reporter of ATM. Li X, Chung CI, Yang J, Chaudhuri S, Munster PN, Shu X. Sci Adv. 2023 Mar;9(9):eade3760. doi: 10.1126/sciadv.ade3760. Epub 2023 Mar 1. 10.1126/sciadv.ade3760 PubMed 36857446
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