ssFv_lenti_wtATMSPARK2
(Plasmid
#195249)
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Purposeexpress wtATMSPARK2 in a lenti vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195249 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonelenti vector
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATMSPARK2 sensor
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Insert Size (bp)2505
- Promoter ssfv
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer ssfv_forward
- 3′ sequencing primer wprer (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ssFv_lenti_wtATMSPARK2 was a gift from Xiaokun Shu (Addgene plasmid # 195249 ; http://n2t.net/addgene:195249 ; RRID:Addgene_195249) -
For your References section:
ATM-SPARK: A GFP phase separation-based activity reporter of ATM. Li X, Chung CI, Yang J, Chaudhuri S, Munster PN, Shu X. Sci Adv. 2023 Mar;9(9):eade3760. doi: 10.1126/sciadv.ade3760. Epub 2023 Mar 1. 10.1126/sciadv.ade3760 PubMed 36857446