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Addgene

pcDNA-mutATMSPARK2
(Plasmid #195248)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195248 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Thr to Ala mutant ATMSPARK2
  • Insert Size (bp)
    2505
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer BGH reversed
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-mutATMSPARK2 was a gift from Xiaokun Shu (Addgene plasmid # 195248 ; http://n2t.net/addgene:195248 ; RRID:Addgene_195248)

  • For your References section:

    ATM-SPARK: A GFP phase separation-based activity reporter of ATM. Li X, Chung CI, Yang J, Chaudhuri S, Munster PN, Shu X. Sci Adv. 2023 Mar;9(9):eade3760. doi: 10.1126/sciadv.ade3760. Epub 2023 Mar 1. 10.1126/sciadv.ade3760 PubMed 36857446
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