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Addgene

pET30a BCP1 NTase
(Plasmid #195245)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195245 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET30a
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5422
  • Total vector size (bp) 6500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BCP1 phage NTase
  • Insert Size (bp)
    1007
  • GenBank ID
    YP_009031408.1
  • Promoter T7
  • Tags / Fusion Proteins
    • SBP (N terminal on insert)
    • His6 (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30a BCP1 NTase was a gift from Douglas Feldman (Addgene plasmid # 195245 ; http://n2t.net/addgene:195245 ; RRID:Addgene_195245)

  • For your References section:

    Bacteriophage antidefense genes that neutralize TIR and STING immune responses. Ho P, Chen Y, Biswas S, Canfield E, Abdolvahabi A, Feldman DE. Cell Rep. 2023 Mar 22;42(4):112305. doi: 10.1016/j.celrep.2023.112305. 10.1016/j.celrep.2023.112305 PubMed 36952342
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