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PurposeMammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195240 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
- Total vector size (bp) 11480
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro
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SpeciesStreptococcus thermophilus
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDAC627 was a gift from Jennifer Doudna (Addgene plasmid # 195240 ; http://n2t.net/addgene:195240 ; RRID:Addgene_195240) -
For your References section:
Precise transcript targeting by CRISPR-Csm complexes. Colognori D, Trinidad M, Doudna JA. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9. 10.1038/s41587-022-01649-9 PubMed 36690762