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Addgene

pDAC446
(Plasmid #195239)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195239 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Total vector size (bp) 11600
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
  • Species
    Streptococcus thermophilus

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDAC446 was a gift from Jennifer Doudna (Addgene plasmid # 195239 ; http://n2t.net/addgene:195239 ; RRID:Addgene_195239)

  • For your References section:

    Precise transcript targeting by CRISPR-Csm complexes. Colognori D, Trinidad M, Doudna JA. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9. 10.1038/s41587-022-01649-9 PubMed 36690762
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