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Addgene

pLKO-cfNHERF1_5
(Plasmid #195226)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195226 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO.1-puro
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NHERF1
  • gRNA/shRNA sequence
    GATCCACCTCCAGTGATAC
  • Species
    canine
  • Entrez Gene
    SLC9A3R1

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO-cfNHERF1_5 was a gift from David Bryant & Keith Mostov (Addgene plasmid # 195226 ; http://n2t.net/addgene:195226 ; RRID:Addgene_195226)

  • For your References section:

    A molecular switch for the orientation of epithelial cell polarization. Bryant DM, Roignot J, Datta A, Overeem AW, Kim M, Yu W, Peng X, Eastburn DJ, Ewald AJ, Werb Z, Mostov KE. Dev Cell. 2014 Oct 27;31(2):171-87. doi: 10.1016/j.devcel.2014.08.027. Epub 2014 Oct 9. 10.1016/j.devcel.2014.08.027 PubMed 25307480
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