GFP-PODXL S481A
(Plasmid
#195223)
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PurposeMammalian expression vector containing GFP tagged Podocalyxin. PKC phosphorylation-deficient mutant.
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195223 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA-GFP-VSVG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePODXL
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SpeciesO. cuniculus (rabbit)
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MutationS481A
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Entrez GenePODXL (a.k.a. PC, PCLP-1, PCLP1)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-PODXL S481A was a gift from David Bryant & Keith Mostov (Addgene plasmid # 195223 ; http://n2t.net/addgene:195223 ; RRID:Addgene_195223) -
For your References section:
A molecular switch for the orientation of epithelial cell polarization. Bryant DM, Roignot J, Datta A, Overeem AW, Kim M, Yu W, Peng X, Eastburn DJ, Ewald AJ, Werb Z, Mostov KE. Dev Cell. 2014 Oct 27;31(2):171-87. doi: 10.1016/j.devcel.2014.08.027. Epub 2014 Oct 9. 10.1016/j.devcel.2014.08.027 PubMed 25307480