PHP.AX
(Plasmid
#195218)
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PurposeAAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 195218 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC57-mini
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSynthetic construct isolate AAV-PHP.AX VP1 gene
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Alt nameAAV-PHP.AX cap
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SpeciesSynthetic
- Promoter p41
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This work supported by NIH BRAIN Initiative grant UF1MH128336
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PHP.AX was a gift from Viviana Gradinaru (Addgene plasmid # 195218 ; http://n2t.net/addgene:195218 ; RRID:Addgene_195218) -
For your References section:
Spatial transcriptomics for profiling the tropism of viral vectors in tissues. Jang MJ, Coughlin GM, Jackson CR, Chen X, Chuapoco MR, Vendemiatti JL, Wang AZ, Gradinaru V. Nat Biotechnol. 2023 Jan 26. doi: 10.1038/s41587-022-01648-w. 10.1038/s41587-022-01648-w PubMed 36702899
