pRevTRE-hygro-GFP-Crumbs3a WT
(Plasmid
#195217)
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PurposeRetroviral Tet response vector containing GFP tagged Crumbs3a
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195217 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRevTRE-hygro-GFP
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Vector typeRetroviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRevTRE-hygro-GFP-Crumbs3a WT was a gift from David Bryant & Ben Margolis (Addgene plasmid # 195217 ; http://n2t.net/addgene:195217 ; RRID:Addgene_195217) -
For your References section:
Trafficking of Crumbs3 during cytokinesis is crucial for lumen formation. Schluter MA, Pfarr CS, Pieczynski J, Whiteman EL, Hurd TW, Fan S, Liu CJ, Margolis B. Mol Biol Cell. 2009 Nov;20(22):4652-63. doi: 10.1091/mbc.e09-02-0137. Epub 2009 Sep 23. 10.1091/mbc.e09-02-0137 PubMed 19776356