B1-INTEGRIN VN
(Plasmid
#195216)
-
PurposeMammalian expression of mCherry tagged B1 Integrin. Autoclustering mutant.
-
Depositing Labs
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 195216 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneUnknown
-
Vector typeMammalian Expression
-
Selectable markersmCherry
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameITGB1
-
SpeciesH. sapiens (human)
-
MutationV737N
-
Entrez GeneITGB1 (a.k.a. CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note there are 3 SNPS in ITGB1- C17G, M170T and N227S. Depositor confirms this does not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
B1-INTEGRIN VN was a gift from David Bryant & Valerie Weaver (Addgene plasmid # 195216 ; http://n2t.net/addgene:195216 ; RRID:Addgene_195216) -
For your References section:
Tensional homeostasis and the malignant phenotype. Paszek MJ, Zahir N, Johnson KR, Lakins JN, Rozenberg GI, Gefen A, Reinhart-King CA, Margulies SS, Dembo M, Boettiger D, Hammer DA, Weaver VM. Cancer Cell. 2005 Sep;8(3):241-54. doi: 10.1016/j.ccr.2005.08.010. 10.1016/j.ccr.2005.08.010 PubMed 16169468