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Addgene

pDA313
(Plasmid #195214)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195214 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Total vector size (bp) 3520
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sfGFP with tetO and a hairpin
  • Species
    Synthetic
  • Insert Size (bp)
    809
  • Promoter T7

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TTGGCCGATTCATTAATGCAGG
  • 3′ sequencing primer GCCCCAAGGGGTTATGCTAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDA313 was a gift from Rahul Sarpeshkar (Addgene plasmid # 195214 ; http://n2t.net/addgene:195214 ; RRID:Addgene_195214)

  • For your References section:

    Rapid modeling of experimental molecular kinetics with simple electronic circuits instead of with complex differential equations. Deng Y, Beahm DR, Ran X, Riley TG, Sarpeshkar R. Front Bioeng Biotechnol. 2022 Sep 28;10:947508. doi: 10.3389/fbioe.2022.947508. eCollection 2022. 10.3389/fbioe.2022.947508 PubMed 36246369
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