GFP-NHERF1 WT
(Plasmid
#195213)
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PurposeMammalian expression vector for GFP tagged NHERF1.
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195213 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-NHERF1 WT was a gift from Anthony Bretscher & David Bryant (Addgene plasmid # 195213 ; http://n2t.net/addgene:195213 ; RRID:Addgene_195213) -
For your References section:
The scaffolding protein EBP50 regulates microvillar assembly in a phosphorylation-dependent manner. Garbett D, LaLonde DP, Bretscher A. J Cell Biol. 2010 Oct 18;191(2):397-413. doi: 10.1083/jcb.201004115. Epub 2010 Oct 11. 10.1083/jcb.201004115 PubMed 20937695