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Addgene

GFP-NHERF1 PDZ1/2*
(Plasmid #195211)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195211 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NHERF1
  • Species
    H. sapiens (human)
  • Mutation
    Dual PDZ1/2 GYGF>GYAA motif mutant
  • Entrez Gene
    NHERF1 (a.k.a. EBP50, NHE-RF, NHERF, NHERF-1, NPHLOP2, SLC9A3R1)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-NHERF1 PDZ1/2* was a gift from Anthony Bretscher & David Bryant (Addgene plasmid # 195211 ; http://n2t.net/addgene:195211 ; RRID:Addgene_195211)

  • For your References section:

    The scaffolding protein EBP50 regulates microvillar assembly in a phosphorylation-dependent manner. Garbett D, LaLonde DP, Bretscher A. J Cell Biol. 2010 Oct 18;191(2):397-413. doi: 10.1083/jcb.201004115. Epub 2010 Oct 11. 10.1083/jcb.201004115 PubMed 20937695
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