P1_Firre_1.49_pDONR221
(Plasmid
#195204)
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PurposePlasmid with pDONR221 backbone containing Firre 1.49 clone. Kanamycin resistance. Gateway cloning system.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195204 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
- Backbone size w/o insert (bp) 4761
- Total vector size (bp) 7461
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Vector typeGateway Cloning Intermediate
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsThe Rinn Lab uses Omnimax 2T1R cells for transformation.
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemFirre 1.49 Clone
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SpeciesM. musculus (mouse)
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Insert Size (bp)2700
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MutationOnly exons
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GenBank ID103012
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
P1_Firre_1.49_pDONR221 was a gift from John Rinn (Addgene plasmid # 195204 ; http://n2t.net/addgene:195204 ; RRID:Addgene_195204) -
For your References section:
Topological organization of multichromosomal regions by the long intergenic noncoding RNA Firre. Hacisuleyman E, Goff LA, Trapnell C, Williams A, Henao-Mejia J, Sun L, McClanahan P, Hendrickson DG, Sauvageau M, Kelley DR, Morse M, Engreitz J, Lander ES, Guttman M, Lodish HF, Flavell R, Raj A, Rinn JL. Nat Struct Mol Biol. 2014 Feb;21(2):198-206. doi: 10.1038/nsmb.2764. Epub 2014 Jan 26. 10.1038/nsmb.2764 PubMed 24463464