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Addgene

pX458-tRNA-SpCas9
(Plasmid #195183)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195183 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX458
  • Total vector size (bp) 9112
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas9
  • Species
    streptococcus pyogenes
  • Insert Size (bp)
    4101
  • Promoter tRNA-Gln

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PciI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer pBRori223
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX458-tRNA-SpCas9 was a gift from Stephanie Roessler (Addgene plasmid # 195183 ; http://n2t.net/addgene:195183 ; RRID:Addgene_195183)
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