pTRIPZ-NUDT17-C-HA-Puro
(Plasmid
#195182)
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PurposeLentiviral vector for DOX-inducible NUDT17 gene expression with C-terminal HA tag. Gateway expression system. Selection by puromycin.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 195182 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTRIPZ
- Backbone size w/o insert (bp) 12383
- Total vector size (bp) 13367
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNUDT17
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SpeciesH. sapiens (human)
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Insert Size (bp)984
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Entrez GeneNUDT17
- Promoter CMV promoter
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pTRIPZ-CMV-F
- 3′ sequencing primer pTRIPZ-UbqC-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTRIPZ-NUDT17-C-HA-Puro was a gift from Stephanie Roessler (Addgene plasmid # 195182 ; http://n2t.net/addgene:195182 ; RRID:Addgene_195182)
Map uploaded by the depositor.
