pDEST26-PPP2CB-C-HA
(Plasmid
#195179)
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PurposeVector for transient expression of PPP2CB gene with C-terminal HA tag, gene flanked by att sites for gateway cloning
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 195179 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDEST26
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Backbone manufacturerInvitrogen TM
- Backbone size w/o insert (bp) 5825
- Total vector size (bp) 6752
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePPP2CB
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Alt namePP2CB
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SpeciesH. sapiens (human)
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Insert Size (bp)927
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GenBank ID
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Entrez GenePPP2CB (a.k.a. PP2Abeta, PP2CB)
- Promoter CMV promoter
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Tag
/ Fusion Protein
- HA (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer M13-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST26-PPP2CB-C-HA was a gift from Stephanie Roessler (Addgene plasmid # 195179 ; http://n2t.net/addgene:195179 ; RRID:Addgene_195179)
Map uploaded by the depositor.
