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siRNA-resistant mNG-ORP8
(Plasmid #195170)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195170 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mNeonGreen-C1
  • Backbone manufacturer
    Allele Biotechnology
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ORP8
  • Alt name
    OSBPL8
  • Alt name
    oxysterol-binding protein-related protein 8 isoform a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2670
  • Mutation
    Wobble mutations for siRNA resistance from K174 to K178
  • Entrez Gene
    OSBPL8 (a.k.a. MST120, MSTP120, ORP8, OSBP10)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mNeonGreen (N terminal on backbone)
    • mNeonGreen (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site SacII (not destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    siRNA-resistant mNG-ORP8 was a gift from Gia Voeltz (Addgene plasmid # 195170 ; http://n2t.net/addgene:195170 ; RRID:Addgene_195170)

  • For your References section:

    An ER phospholipid hydrolase drives ER-associated mitochondrial constriction for fission and fusion. Nguyen TT, Voeltz GK. Elife. 2022 Nov 30;11:e84279. doi: 10.7554/eLife.84279. 10.7554/eLife.84279 PubMed 36448541
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