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Addgene

mNG-Mfn1
(Plasmid #195162)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195162 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mNeonGreen-N1
  • Backbone manufacturer
    Allele Biotechnology
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mfn1
  • Alt name
    Mitofusin-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2229
  • Entrez Gene
    MFN1 (a.k.a. hfzo1, hfzo2)
  • Promoter CMV
  • Tag / Fusion Protein
    • mNeonGreen (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mNG-Mfn1 was a gift from Gia Voeltz (Addgene plasmid # 195162 ; http://n2t.net/addgene:195162 ; RRID:Addgene_195162)

  • For your References section:

    An ER phospholipid hydrolase drives ER-associated mitochondrial constriction for fission and fusion. Nguyen TT, Voeltz GK. Elife. 2022 Nov 30;11:e84279. doi: 10.7554/eLife.84279. 10.7554/eLife.84279 PubMed 36448541
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