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Addgene

siRNA-resistant ABHD16A-mCherry
(Plasmid #195161)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195161 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mCherry-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ABHD16A
  • Alt name
    Phosphatidylserine lipase ABHD16A, isoform 1
  • Alt name
    BAT5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1674
  • Mutation
    Wobble mutations for siRNA resistance from L90 to P95
  • Entrez Gene
    ABHD16A (a.k.a. BAT5, D6S82E, NG26, PP199, SPG86, hBAT5)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site SacII (not destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS results found an early stop codon in insert ORF, but it will not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    siRNA-resistant ABHD16A-mCherry was a gift from Gia Voeltz (Addgene plasmid # 195161 ; http://n2t.net/addgene:195161 ; RRID:Addgene_195161)

  • For your References section:

    An ER phospholipid hydrolase drives ER-associated mitochondrial constriction for fission and fusion. Nguyen TT, Voeltz GK. Elife. 2022 Nov 30;11:e84279. doi: 10.7554/eLife.84279. 10.7554/eLife.84279 PubMed 36448541
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